Dylight 550 vs pe

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August undefined, 2024

WebAnti-Human Fc DyLight® 550 secondary antibody preadsorbed validated for ICC/IF, Flow Cyt. Cited in peer-reviewed publications. Other DyLight® 550 secondaries available. ... Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC HRP SureLight® PE Unconjugated Unconjugated. You may also be interested in. Primary . … WebH9 human embryonic stem (ES) cells were stained with either PE Mouse Anti-SSEA-4 (solid line) or PE mouse IgG3 (A112-3) isotype control (catalog number 559926, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System.

Antibodies Conjugated to Fluorophores Bio-Rad

WebGoat IgG (2mg/mL) was reacted with a 15-fold molar excess of DyLight 550 NHS Ester (Part No. 62262). A. Measure A. 280. and A. max. of the DyLight 550-conjugated Goat IgG . 1. The final labeling reaction solution was dialyzed overnight to remove excess, nonconjugated dye. 2. The dialyzed conjugate was diluted 1/10 in phosphate buffered … WebDyLight® Dyes. DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy. Newborn rat primary cardiac myocytes stained with mouse anti-desmin, detected with goat anti-mouse IgG:DyLight® 488 (STAR74D488); rat anti-alpha tubulin detected with goat anti-rat IgG:DyLight® 649 (STAR71D649); and rabbit anti ... pho bac menu atl

Which fluorophores are useful for flow cytometry - Bio-Rad

WebBoster Bio Anti-Human CD2AP DyLight® 550 conjugated Antibody catalog # A01756-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human. … WebBoth the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation. Our catalog includes secondary antibodies conjugated with DyLight ® 488, DyLight ® 550, DyLight ® 594 and DyLight® 650. Fig 1. pho bac red boat

Fluorochrome Guide - Leinco Technologies

DyLight® Fluorochrome conjugated secondary antibodies Abcam

Web38 rows · PE/Cyanine5: 565: 670: 660/20: 5: PE/Dazzle™ 594: 565: 610: 610/20: 5: PE/Fire™ 700* 565: 695: 670/30: 5: Brilliant Violet 421™ ... Spark Blue™ 550* 516: 540 … WebInvitrogen Dylight Antibody Labeling kits provide everything needed to label 1 mg of antibody or protein with amine-reactive Dylight dye and subsequent free dye removal. Choose from a variety of Dylight fluorophores across the spectrum to covalently label your antibody or protein. Labeled antibodies are typically ready to use in 90 minutes with ... pho bac irvineWebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … pho bac hoa viet madison

"WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. DyLight dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio ... " - Dylight 550 vs pe

Dylight 550 vs pe

DyLight™ 550 Maleimide - Thermo Fisher Scientific

WebDyLight 550 562 576 4 PE* 496, 546 578 5 APC 650 661 4 Alexa Fluor 647 650 665 4 DyLight 650 654 673 4 PerCP 490 675 2 Alexa Fluor 700 Infrared 702 723 2 * PE is the … WebBackground. CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface …

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WebJan 26, 2012 · IHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments …

WebDyLight 550 561 562 576 FL3 FL2 FL2 DyLight 550 PE 488, 561 496, 565 578 FL3 FL3 FL2 FL2 FL2 FL2 PE ... PE-Cy5 488, 561 496, 565 670 FL4 FL4 FL4 FL4 FL3 FL2 PE … WebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 650 fluorochrome? The excitation is 652nm and the emission is 672nm. As a comparison, the excitation of DyLight® 649 was 654nm and the emission was 673nm. 5.

WebDyLight 550 is brighter in most applications than TRITC, Cy3 & AlexaFluor 555. All Dylight 550 ... R-PE (Ex Max 496 nm / Em. Peak 578nm) R-phycoerythrin (R-PE) has a … WebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 550 fluorochrome? The excitation is 562nm and the emission is 576nm. As a comparison, the excitation of DyLight® …

WebStreptavidin conjugated to Dylight®550 quantity ... Streptavidin: SureLight® B-PE;1mg. Binding Moiety:?Streptavidin Recommended Dilutions: IHC 1:100;? WB 1:1000 $ 279.00. #D4-2212. Add to cart Details; SureLight® Streptavidin R-Phycoerythrin (standard-intensity) Important Features of SureLight? SAPE (standard-intensity) Interchangeable with ...

WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … pho bac hoa viet south sacWeb10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. Labeling Reaction . 1. To protect reagents from moisture, allow DyLight NHS Esters and DMF to equilibrate to room temperature before opening vials. 2. pho bac hoa viet south sac reviewWebDyLight 550 561 562 576 FL3 FL2 FL2 DyLight 550 PE 488, 561 496, 565 578 FL3 FL3 FL2 FL2 FL2 FL2 PE ... PE-Cy5 488, 561 496, 565 670 FL4 FL4 FL4 FL4 FL3 FL2 PE-Cy5 DyLight 650 640 652 672 FL4 FL4 FL3 DyLight 650 PerCP 488 482 676 FL4 FL4 FL4 FL4 FL3 FL2 PerCP PerCP-Cy5.5 488 482 690 FL4 FL4 FL4 FL4 FL3 FL2 PerCP-Cy5.5 pho b.a.e. southlakeWebHiLyte FluorTM 555 550 566 5-TAMRA 541 568 Alexa Fluor®546 556 573 532 DyLight®550 562 576 Phycoerythrin (PE) 496,566 576 488 Tetramethyl Rhodamine Isothiocyanate (TRITC) 557 576 EviTag TM quantum dots-Birch Yellow 560 580 Cy3.5® … pho bac seattle historyWebEach Thermo Scientific DyLight antibody labeling kit contains all the necessary components for three 1-mg labeling reactions of any protein with a molecular weight of 50-150 kDa, … pho bac garlandWebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … pho bac sup shop turmeric photWebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View Available Formulations. Catalog# & … phobaeticus magnus size